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Brief Biography Dr Graham received his honours degree in Biochemistry from The Queen’s University of Belfast (1996). Subsequently he undertook a Masters of Medical Science at the same institution where his research focused upon the analysis of the effects of the indole amine reserpine on vesicular monoamine transporters (1998). Dr Graham continued his doctoral studies at the University of Ulster within the Pharmaceutical Biotechnology Research Group being awarded his PhD in 2003. This work led to the isolation and identification of the first bradykinin inhibitory peptide identified from snake venoms (Graham et al 2005b). Research focus Dr Graham is currently a senior postdoctoral researcher within the European Union funded Northern Ireland Centre of Excellence in Functional Genomics. His work focuses upon the application of mass spectrometry, biochemistry and proteomics for biomarker identification of micro-organisms that are involved in infectious disease, emerging nosocomial pathogens, extremophiles as well as cystic fibrosis’, cancer, eye disease and the identification of pharmaceutical agents from natural sources. He is responsible for design and establishment of the proteomic facility at the University of Ulster, allowing both gel-free and gel-based multidimensional bottom-up proteomics to be carried out. The facility comprises, a nano LC-MS/MS (Applied Biosystems Hybrid triple quadrupole ion trap 3200 QTRAP and LC Packings Dionex Ultimate 3000 HPLC), in addition there is a multi perfusion chemistry protein separation Biocad Vision Workstation™ an EXQuest™ spot cutter with integrated 2D-PAGE and DiGE PDQuest analysis software. The suite also has a number of other facilities for global proteomic analysis; including label free (emPAI) and label based (iTRAQ) protocols and experimental work flows for gel free quantitative proteomic analysis and post-translational modification analyses of proteins. Dr Graham is an editorial board member for the journal Proteomic Insights. He was an invited speaker at the International symposium on Extremophiles in Japan (2005) and has written an invited review on mass spectrometry and proteomics for Microbial Cell Factories. He is an invited reviewer for a number of high impact peer review journals that include: the Journal of Proteome Research, the Journal of Proteomics, Proteomics, Proteomics-Clinical applications and the Journal of Cellular and Molecular Life Sciences. He has also been invited to review a Proteomic book proposal by the scientific publishing group Taylor & Francis and a Systems Biology book proposal by Wiley & Sons publishing group. In addition, he was recently an invited group leader at the Medical innovations Forum, London, advances in proteomics in medicine/ Selected Publications: Graham RL, Graham C, Theakston D, McMullan G, Shaw C; Elucidation of trends within venom components from the snake families Elapidae and Viperidae using gel filtration chromatography; Toxicon, 51: 121-129, 2008 Graham RL, Pollock CE, O’Loughlin SN, Ternan NG, Weatherly DB, Jackson PJ, Tarleton RL, McMullan G; Multidimensional analysis of the insoluble sub-proteome of Oceanobacillus iheyensis HTE831, an alkaliphilic and halotolerant deep-sea bacterium isolated from the Iheya ridge; Proteomics; 7: 82-91, 2007 Graham RL, O’Loughlin SN, Pollock CE, Ternan NG, Weatherly DB, Jackson PJ, Tarleton RL, McMullan G; A combined shotgun and multidimensional proteomic analysis of the insoluble subproteome of the obligate thermophile, Geobacillus thermoleovorans T80; Journal of Proteome Research, 5: 2465–2473, 2006 Graham RL, Pollock CE, Ternan NG, McMullan G; Top-Down Proteomic Analysis of the Soluble Sub-Proteome of the Obligate Thermophile, Geobacillus thermoleovorans T80: Insights into Its Cellular Processes; Journal of Proteome Research, 5: 822– 828, 2006 Graham RL, Pollock CE, O’Loughlin SN, Ternan NG, Weatherly DB, Jackson PJ, Tarleton RL, McMullan G; Multidimensional proteomic analysis of the soluble subproteome of the emerging nosocomial pathogen Ochrobactrum anthropi; Journal of Proteome Research, 5: 3145-3153, 2006 O’Loughlin SN, Graham RL, McMullan G, Ternan NG; A role for carbon catabolite repression in the metabolism of phosphonoacetate by Agromyces fucosus Vs2; FEMS Microbiology Letters; 261: 133-140, 2006 Graham RL, Graham C, McClean S, Chen T, O’Rourke M, Hirst D, Theakston D, Shaw C; Identification and functional analysis of a novel bradykinin inhibitory peptide in the venoms of New World Crotalinae pit vipers; Biochemical and Biophysical Research Communications, 338: 1587-1592, 2005 Graham RL, McClean S, O’Kane EJ, Theakston D, Shaw C; Adenosine in the venoms from viperinae snakes of the genus Bitis: identification and quantitation using LC/MS and CE/MS; Biochemical and Biophysical Research Communications, 333: 88-94, 2005 For further information please contact: |
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