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Contact Details:Tel: 028 7032 4406 Fax: 028 7032 4375 Email: s.mcclean@ulster.ac.uk _ Brief Biography:Dr Stephen McClean received a BSc degree in Science with Business from the University of Ulster in 1990. He stayed at the University of Ulster to complete Masters of Research and PhD degrees in 1996 and 1999, respectively, majoring in analytical chemistry. In 1990, he took obtained a post-doctoral research position in bio-analytical chemistry studying amphibian skin secretions and reptile venoms using modern analytical techniques in particular mass spectrometry. Stephen was appointed to his current position of Lecturer in Proteomics at the University of Ulster in December 2001. Dr McClean is a member of the Royal Society of Chemistry. Main Research Interests:The isolation and characterisation of bioactive peptides and small molecules from amphibian skin secretions and reptile and arachnid venoms. This is done by QTOF MS/MS de novo sequencing of peptides, Edman degradation sequencing, FMOC solid phase peptide synthesis and MALDI-TOF mass spectrometry. The quantitation of licit and illicit drug compounds in human hair samples by chromatographic and mass spectrometric methods is also of interest. Research Publications (since 2000):Doherty B, Rodriguez V, Leslie JC, McClean S, Smyth WF; An electrospray ionisation tandem mass spectrometric investigation of selected psychoactive pharmaceuticals and its application in drug and metabolite profiling by liquid chromatography/electrospray ionisation tandem mass spectrometry; Rapid Communications in Mass Spectrometry, 21: 2031-2038, 2007 Thompson AH, Bjourson AJ, Orr DF, Shaw C and McClean S; A combined mass spectrometric and cDNA sequencing approach to the isolation and characterisation of novel antimicrobial peptides from the skin secretions of Phyllomedusa hypochondrialis azurea; Peptides, 28: 1331–1343, 2007 Thompson AH, Bjourson AJ, Orr DF, Shaw C and McClean S; Amphibian skin secretomics: application of parallel quadrupole time-of-flight mass spectrometry and peptide precursor cDNA cloning to rapidly characterize the skin secretory peptidome of Phyllomedusa hypochondrialis azurea: discovery of a novel peptide family, the hyposins; Journal of Proteome Research, 6: 3604-3613, 2007 Zhou J, Bjourson AJ, Coulter DJ, Chen T, Shaw C, O’rourke M, Hirst DG, Zhang Y, Rao P, McClean S; Bradykinin-related peptides, including a novel structural variant, (Val1)-bradykinin, from the skin secretion of Guenther’s frog, Hylarana guentheri and their molecular precursors; Peptides, 28: 781-789, 2007 Graham C, Richter SC, McClean S, O’Kane E, Flatt PR, Shaw C; Histamine-releasing and antimicrobial peptides from the skin secretions of the dusky gopher frog, Rana sevosa; Peptides, 27:1313-1319, 2006 Smyth WF, Leslie JC, McClean S, Hannigan BM, McKenna HP, Doherty B, Joyce C and O’Kane E; The characterisation of selected antidepressant drugs using electrospray ionisation with ion trap mass spectrometry and with quadrupole time of flight mass spectrometry and their determination by HPLC-ESI-MSn; Rapid Communications in Mass Spectrometry, 20:1637-1642, 2006 Thompson AH, Bjourson AJ, Shaw C and McClean S; Bradykinin-related peptides from Phyllomedusa hypochondrialis azurea: mass spectrometric structural characterisation and cloning of precursor cDNAs; Rapid Communications in Mass Spectrometry, 20: 3780–3788, 2006 Zhou Jianwu, McClean Stephen, Thompson Alan, Zhang Yang, Shaw Chris, Rao Pingfan, Bjourson Anthony J; Purification and characterization of novel antimicrobial peptides from the skin secretion of Hylarana guentheri; Peptides, 27, 3077-3084, 2006 Graham RLJ, Graham C, McClean S, Chen T, O’Rourke M, Hirst D, Theakston D, Shaw C; Identification and functional analysis of a novel bradykinin inhibitory peptide in the venoms of New World Crotalinae pit vipers; Biochemical and Biophysical Research Communications, 338: 1587-1592, 2005 Graham RLJ, McClean S, O’Kane EJ, Theakston D and Shaw C; Adenosine in the venoms from viperinae snakes of the genus Bitis: identification and quantitation using LC/MS and CE/MS; Biochemical and Biophysical Research Communications, 333: 88-94, 2005 Graham C, Irvine AE, McClean S, Richter S, Flatt PR and Shaw C; Peptide Tyrosine Arginine, a potent immunomodulatory peptide isolated and structurally characterized from the skin secretions of the dusky gopher frog, Rana sevosa; Peptides, 26: 737-743, 2005 Zheng HR, Ojha PC, McClean S, Graham C, Black ND, Hughes JG, Shaw C; A computational approach for peptidomic analysis in taxonomic study of amphibian species; IEEE Transactions on Nanobioscience, 4: 241-247, 2005 Chen T, Orr DF, O’Rourke M, McGlynn C, Bjourson AJ, McClean S, Hirst DG, Rao P and Shaw C: Pachymedusa dacnicolor tryptophyllin-1 (PdT-1): structural characterization, pharmacological activity and cloning of precursor cDNA; Regulatory Peptides, 117: 25-32, 2004 Marenah L, Orr DF, McClean S, Shaw C, Flatt PR and Abdel-Wahab YHA; Skin secretions of the toad Bombina variegata frog contain multiple Insulin releasing peptides including bombesin and entirely novel insulinotropic structures; Biological Chemistry, 385: 315-321, 2004 Marenah L, Orr DF, McClean S, Flatt PR, Shaw C and Abdel-Wahab YHA; Brevinin-1 and multiple insulin-releasing peptides in the skin of the frog Rana palustris; Journal of Endocrinology, 181: 347-354, 2004 Marenah L, McClean S, Flatt PR, Orr DF, Shaw C, Abdel-Wahab YH; Novel insulin-releasing peptides in the skin of Phyllomedusa trinitatis frog include 28 amino acid peptide from dermaseptin BIV precursor; Pancreas, 29:110-115, 2004 Marenah L, Shaw C, Orr DF, McClean S, Flatt PR, Abdel-Wahab YH; Isolation and characterisation of an unexpected class of insulinotropic peptides in the skin of the frog Agalychnis litodryas; Regulatory Peptides, 120: 33-38, 2004 Zheng H, Ojha PC, McClean S, Black ND, Hughes JG and Shaw C; Heuristic charge assignment for deconvolution of electrospray ionization mass spectra; Rapid Communications in Mass Spectrometry, 17: 429-436, 2003 Chen T, Bjourson AJ, McClean S, Orr DF, O’Kane EJ, Rao P and Shaw C; Cloning of maximakinin precursor cDNAs from Chinese toad, Bombina maxima, venom; Peptides, 24: 853 – 861, 2003 Zheng H, Ojha PC, McClean S, Black ND, Hughes JG and Shaw C; Heuristic charge assignment for deconvolution of electrospray ionization mass spectra; Rapid Communications in Mass Spectrometry, 17: 429-436, 2003 Chen T, Orr DF, Bjourson AJ, McClean S, O’Rourke M, Hirst DG, Rao P and Shaw C; Novel bradykinins and their precursor cDNAs from the European Yellow Bellied Toad (Bombina variegata) skin; European Journal of Biochemistry, 269: 4693-4700, 2002 Chen T, Orr DF, Bjourson AJ, McClean S, O’Rourke M, Hirst DG, Rao P, Shaw C; Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis); Peptides, 23:1547-1555, 2002 Rahman KS, Rahman TJ, McClean S, Marchant R, Banat IM; Rhamnolipid biosurfactant production by strains of Pseudomonas aeruginosa using low-cost raw materials; Biotechnology Progress, 18: 1277-1281, 2002 McClean S, Robinson RC, Shaw C, Smyth WF; Characterisation and determination of indole alkaloids in frog-skin secretions by electrospray ionisation ion trap mass spectrometry; Rapid Communications in Mass Spectrometry, 16: 346-354, 2002 McClean S, O’Kane EJ, Smyth WF; The identification and determination of selected 1,4-benzodiazepines by an optimised capillary electrophoresis-electrospray mass spectrometric method; Electrophoresis, 21:1381-1389, 2000 McClean S, O’Kane EJ, Smyth WF; Electrospray ionisation-mass spectrometric characterisation of selected anti-psychotic drugs and their detection and determination in human hair samples by liquid chromatography-tandem mass spectrometry; Journal of Chromatography B: Biomedical Sciences and Applications, 740: 141-157, 2000 Smyth WF, McClean S, Ramachandran VN; A study of the electrospray ionisation of pharmacologically significant 1,4-benzodiazepines and their subsequent fragmentation using an ion-trap mass spectrometer; Rapid Communications in Mass Spectrometry, 14: 2061-2069, 2000 For further information please contact: |
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