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Contact Details:Tel: 028 7032 4427 Fax: 028 7032 4375 Email: jsg.dooley@ulster.ac.uk _ Brief Biography:Professor James Dooley obtained a BSc in Microbiology from the National University of Ireland (Galway) in 1982 and a PhD in Biochemistry from the University of Victoria in 1988. He moved to the University of Ulster in 1990 to take up a Lectureship post following a period of time at the Moredun Research Institute in Edinburgh where he was undertaking research into improved diagnostics of animal diseases. Main Research Interests:Professor Dooley’s current research interests are centred on developing a greater understanding of the biology of human and animal pathogens, for example: Molecular techniques for diagnostic microbiology and epidemiology, with a major focus on difficult to culture pathogens, particularly the protozoan parasite Cryptosporidium parvum. Pathogenic mechanisms of Cryptosporidium species. Bacterial population analysis based on ribosomal RNA sequence determination. Role of protozoa in the ecology of pathogenic bacteria with particular emphasis on Campylobacter species. Bacterial biofilm production by respiratory pathogens with emphasis on Pseudomonas aeruginosa. Structure and function relationships in outer membrane components of gram negative bacterial pathogens with a particular emphasis on regular surface protein arrays in Aeromonas species. Professor Dooley is applying a variety of technologies to his research activities, including Laser Capture Microscopy for improved diagnosis and molecular epidemiology of a variety of pathogens that are difficult to culture. Research Project Focus:One of the research projects in which he is currently engaged is: Antibiotic resistant bacteria in Irish waters (which has been funded by the Environmental Protection Agency). This research seeks to investigate the role of water in disseminating antibiotic resistant enterococci. The enterococci are isolated from environmental samples by laboratory cultivation. Sampling sites are located in two instrumented headwater catchments of the River Blackwater system, which drains into Lough Neagh, Ireland’s largest inland lake. Many characteristics for both catchments have been well documented as part of the ongoing Blackwater TRACE project. The extensive database available comprises data on soil fertility, septic systems and farmyard infrastructure. Molecular epidemiology tools specifically, Multilocus Sequence Typing (MLST), are being used to compare isolates of enterococci recovered on farms and in the aquatic environment. Research Publications (from 2000):Maeda Y, Loughrey A, Earle JA, Millar BC, Rao JR, Kearns A, McConville O, Goldsmith CE, Rooney PJ, Dooley JS, Lowery CJ, Snelling WJ, McMahon A, McDowell D, Moore JE; Antibacterial activity of honey against community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA); Complementary Therapies in Clinical Practice, 14: 77-82, 2008 Snelling WJ, Stern NJ, Lowery CJ, Moore JE, Gibbons E, Baker C, Dooley JS Colonization of broilers by Campylobacter jejuni internalized within Acanthamoeba castellanii; Archives of Microbiology, 189: 175-179, 2008 Stirling J, Griffith M, Dooley JS, Goldsmith CE, Loughrey A, Lowery CJ, McClurg R, McCorry K, McDowell D, McMahon A, Millar BC, Rao J, Rooney PJ, Snelling WJ, Matsuda M, Moore JE; Zoonoses Associated with Petting Farms and Open Zoos; Vector Borne Zoonotic Diseases, 8: 85-92, 2008 Stirling J, Griffith M, Blair IM, Cormican M, Dooley JS, Goldsmith CE, Glover SG, Loughrey A, Lowery CJ, Matusuda M, McClurg R, McCorry K, McDowell D, McMahon A, Millar BC, Rao J, Rooney PJ, Snelling WJ, Xu J, Moore JE; Prevalence of gastrointestinal bacterial pathogens in a population of zoo animals; Zoonoses and Public Health, 55: 166-172, 2008 Xu J, Stanley T, Millar BC, McClurg R, Shaw A, Crothers L, Goldsmith CE, Rooney PE, Loughrey A, Murphy PG, Dooley JSG, Moore JE; Difficult-to-identify bacteria: how use of 16S rDNA PCR and gene sequencing can help; British Journal of Biomedical Sciences, 65: 33-36, 2008 Graham JE, Moore JE, Jiru X, Moore JE, Goodall EA, Dooley JS, Hayes VE, Dartt DA, Downes CS, Moore TC; Ocular Pathogen or Commensal: A PCR-Based Study of Surface Bacterial Flora in Normal and Dry Eyes; Investigations in Ophthalmology and Vision Science, 48: 5616-5623, 2007 Maeda Y, Kenny F, Coulter WA, Loughrey A, Nagano Y, Goldsmith CE, Millar BC, Dooley JS, Lowery CJ, Rooney PJ, Matsuda M, Moore JE; Bactericidal activity of denture cleaning formulations against planktonic healthcare-associated and community-associated methicillin-resistant Staphylococcus aureus (MRSA); American Journal of Infection Control, 35: 619-622, 2007 Moore JE, Nagano Y, Millar BC, McCalmont M, Elborn JS, Rendall J, Pattison S, Dooley JS, Goldsmith CE; Environmental persistence of Pseudomonas aeruginosa and Burkholderia multivorans in sea water: preliminary evidence of a viable but non-culturable state; British Journal of Biomedical Science, 64:129-131, 2007 Nagano Y, Finn MB, Lowery CJ, Murphy T, Moriarty J, Power E, Toolan D, O’loughlin A, Watabe M, McCorry KA, Crothers E, Dooley JS, Rao JR, Rooney PJ, Millar BC, Matsuda M, Elborn JS, Moore JE; Occurrence of Cryptosporidium parvum and Bacterial Pathogens in Faecal Material in the Red Fox (Vulpes vulpes) Population; Veterinary Research Communications, 31: 559-564, 2007 Panas P, McMullan G and Dooley JSG; RT-TGGE as a guide for the successful isolation of phosphonoacetate degrading bacteria; Journal of Applied Microbiology, 103: 237-244, 2007 Shigematsu M, Nagano Y, Millar BC, Kenny F, Lowery CJ, Xiao L, Rao JR, Nicholson V, Watabe M, Heaney N, Sunnotel O, McCorry K, Rooney PJ, Snelling W, Dooley JS, Elborn JS, Matsuda M, Moore JE; Molecular detection and identification of Cryptosporidium species in lettuce employing nested small-subunit rRNA PCR and direct automated sequencing; British Journal of Biomedical Science, 64:133-135, 2007 Snelling WJ, Lin Q, Moore JE, Millar BC, Tosini F, Pozio E, Dooley JS, Lowery CJ; Proteomic analysis and protein expression during sporozoite excystation of Cryptosporidium parvum (coccidia, Apicomplexa); Molecular and Cellular Proteomics,. 6: 346-55, 2007 Sunnotel O, Snelling WJ, McDonough N, Browne L, Moore JE, Dooley JS, Lowery CJ; The effectiveness of standard UV depuration at inactivating Cryptosporidium parvum recovered from spiked pacific oysters (Crassostrea gigas); Applied and Environmental Microbiology, 73: 5083-5087, 2007 Thompson HP, Dooley JS, Kenny J, McCoy M, Lowery CJ, Moore JE, Xiao L; Genotypes and subtypes of Cryptosporidium spp. in neonatal calves in Northern Ireland; Parasitology Research, 100: 619-624, 2007 Wishart K, Loughrey A, McClurg RB, Goldsmith CE, Millar BC, Rao J, Sengupta B, Dooley JSG, Rooney PJ, Moore JE; Lack of horizontal gene transfer of methicillin-resistance genetic determinants from PBP2a-positive coagulase-negative staphylococci (CNS) to methicillin-sensitive Staphylococcus aureus (MSSA) employing transcutaneous electrical nerve stimulation (TENS); British Journal of Biomedical Science, 64: 6-9, 2007 Yuan I, Xu J, Millar BC, Dooley JS, Rooney PJ, Alexander HD, Moore JE; Molecular identification of environmental bacteria in indoor air in the domestic home: Description of a new species of Exiguobacterium; International Journal of Environmental Health Research, 17: 75-82, 2007 Kourkoutas Y, Kandylis P, Panas P, Dooley JSG, Nigam P, and Koutinas AA; Evaluation of Freeze-Dried Kefir Co-Culture as Starter in Feta-Type Cheese Production; Applied and Environmental Microbiology, 72: 6124-6135, 2006 Moore JE, Matsuda M, Sekizuka T, Fanning S, Murayama O, Yokoi T, Kagawa S, Nagano K, Shimura H, Watabe M, Nagano Y, Usui K, Imamaki R, Aritomi T, Harada C, Iida H, Mitsuhashi N, Miyatake T, Shigematsu M, Rao JR, Lowery CJ, Millar BC, Dooley JSG and Rooney PJ; Subspecies characterization of urease-positive thermophilic Campylobacter (UPTC) isolated from shellfish employing modified flagellin (flaA) restriction fragment length polymorphism (RFLP) typing; Journal of Shellfish Research, 25: 625-629, 2006 Panas P, Ternan NG, Dooley JSG, and McMullan G; Detection of phosphonoacetate degradation and phnA genes in soil bacteria from distinct geographical origins suggest its possible biogenic origin; Environmental Microbiology, 8: 939-945, 2006 Snelling WJ, McKenna JP, Hack CJ, Moore JE, and Dooley JSG; An examination of the diversity of a novel Campylobacter reservoir; Archives of Microbiology, 186: 31-40, 2006 Sunnotel O, Snelling WJ, Xiao L, Moule K, Moore JE, Millar BC, Dooley JSG, and Lowery CJ; The rapid and sensitive detection of single Cryptosporidium oocysts from archived glass slides; Journal of Clinical Microbiology, 44: 3285-3291, 2006 Xiao L, Moore JE, Ukoh U, Gatei W, Lowery CJ, Murphy TM, Dooley JS, Millar BC, Rooney PJ, Rao JR; Prevalence and identity of Cryptosporidium spp. in pig slurry; Applied and Environmental Microbiology, 72: 4461-4463, 2006 Moore JE, Corcoran D, Dooley JSG, Fanning S, Lucey B, Matsuda M, McDowell DA, Megraud F, Millar BC, O’Mahony R, O’Riordan L, O’Rourke M, Rao JR, Rooney PJ, Sails A, Whyte P; Campylobacter; Veterinary Research, 36: 351-382 2005 Sharkey FH, Dooley JS, Haylock RW; Quantitative Effects of Carbohydrates and Aromatic Amino Acids on Clostridium botulinum Toxin Gene Expression Using a Rapid Competitive RT/PCR Assay; Journal of Molecular Microbiology and Biotechnology, 9: 35-43, 2005 Snelling WJ, McKenna JP, Lecky DM, Dooley JSG; Survival of Campylobacter jejuni in waterborne protozoa; Applied and Environmental Microbiology, 71: 5560-5571, 2005 Moore JE, Shaw A, Howard Jennifer L, Dooley JSG and Elborn JS; Infection control and the significance of sputum and other respiratory secretions from adult patients with cystic fibrosis; Annals of Clinical Microbiology and Antimicrobials, 3: 8, 2004 Russell DA, Dooley JS, Haylock RW; The steady-state orgA specific mRNA levels in Salmonella enterica serovar Typhimurium are repressed by oxygen during logarithmic growth phase but not early-stationary phase; FEMS Microbiology Letters, 236: 65-72, 2004 Watabe M, Sekizuka T, Millar BC, Rooney PJ, Moore JE, Matsuda M, Xiao LH, Lowery CJ, Dooley JSG, Rao JR; Investigation of faecal pathogens in a flock of mute swans (Cygnus olor); Veterinary Record, 154: 279-280, 2004 Cunningham, AM, Finn, MB, Lowery CJ, Dooley JSG and Moore JE; Detection and sensitivity analysis of Cryptosporidium parvum oocysts in the shellfish Mytilus edulis using Nested PCR of the 18S rRNA gene; Environmental Scientist, 12: 18-21, 2003 Millar BC, Lowery CJ, Xu JR, Dooley JSG, Moore JE; Sequence analysis of partial regions of the 5.8S rRNA internal transcribed region 2 and 28S rRNA of Isospora belli; British Journal of Biomedical Science, 60: 114-116, 2003 Watabe M, Rao JR, Stewart TA, Xu J, Millar BC, Xiao L, Lowery CJ, Dooley JSG, Moore JE; Prevalence of bacterial faecal pathogens in separated and unseparated stored pig slurry; Letters in Applied Microbiology, 36: 208-212, 2003 Glaberman S, Moore JE, Lowery CJ, Chalmers R, Sulaimah I, Elwin K, Rooney PJ, Millar BC, Dooley JSG, Lal AA, and Xiao L; Investigation of three drinking water associated outbreaks of Cryptosporidiosis in Northern Ireland using genotyping and subgenotyping tools; Emerging Infectious Diseases, 8: 631-633, 2002 McClurg KR, McClurg RB, Moore JE, Dooley JSG; Efficient isolation of campylobacters from stools: what are we missing? Journal of Clinical Pathology, 55: 239-240, 2002 Millar BC, Lowery CJ, Xu J, Dooley JS and Moore JE; Sequence analysis of partial regions of the 5.8S rRNA – internal transcribed region 2 and 28S rRNA of Ispora belli; British Journal of Biomedical Science, 60:114-116, 2002 Boost MV, O’Donoghue MM, Dooley JS; Prevalence of carriage of antimicrobial resistant strains of Streptococcus pneumoniae in primary school children in Hong Kong; Epidemiology and Infection, 127:49-55, 2001 Lowery CJ, Millar BC, Moore JE, Xu J, Xiao L, Rooney PJ, Crothers L and Dooley JSG; Molecular genotyping of human cryptosporidiosis in Northern Ireland: epidemiological aspects and review; Irish Journal of Medical Science, 170: 246-250, 2001 Lowery CJ, Nugent P, Moore JE, Millar BC, Xiru X, and Dooley JSG; PCR-IMS detection and molecular typing of Cryptosporidium parvum recovered from a recreational river source and an associated mussel (Mytilus edulis) bed in Northern Ireland; Epidemiology and Infection, 127: 545-553, 2001 Lowery CJ, Moore JE, Millar BC, McCorry KAJ, Xu J, Rooney PJ, Dooley JSG; Occurrence and molecular genotyping of Cryptosporidium spp. in surface waters in Northern Ireland; Journal of Applied Microbiology, 91:774-779, 2001 Millar C, Moore J, Lowery C, McCorry K, Dooley J; Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple methods suited for outbreak analysis; International Journal of Hygiene and Environmental Health, 204:191-194, 2001 Lowery CJ, Moore JE, Millar BC, Burke DP, McCorry KAJ, Crothers E, et al; Detection and speciation of Cryptosporidium spp. in environmental water samples by immunomagnetic separation, PCR and endonuclease restriction; Journal of Medical Microbiology, 49: 779-785, 2000 McGrath S, Dooley JSG, Haylock RW; Quantification of Clostridium botulinum toxin gene expression by competitive reverse transcription-PCR; Applied and Environmental Microbiology, 66: 1423-1428, 2000 For further information please contact: |
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